Syringe & Haemocytometer and Microtome

Syringe & Haemocytometer and Microtome

Syringe:

The syringe is very important instrument, generally use liquid substance push and collection form human body.

Types of Syringe:

1. All glass syringe
2. Disposable
3. Lumber puncture

Parts of syringe:

1. Piston
2. Barrel
3. Plunger
4. Nozzle
5. Needle
6. Needle cover

Haemocytometer:

Haemocytometer box is in apparatus which based in detection of total count of WBC, RBC, platelets, circulatory eosinophil.

Consists of:

1. RBC pipette
2. WBC pipette
3. Special cover slip
4. Counting chamber

Use of Haemocytometer :

1. Total count of WBC
2. Total count of RBC
3. Total count of platelets
4. Total count of circulatory eosinophil.

Microtome:

Microtome is machine designed to cut thin sections of human, animal or plant tissue.

For kinds of microtome:

1. Rotary microtome : For cutting paraffin-embedded sections.
2. Slide microtome : For cutting colliding –embedded section.
3. Freezing microtome : For cutting unimpeded  tissues.
4. Cold microtome: for cutting thin frozen section of unimpeded fresh or fixed tissue

Major parts of microtome:

1. The block holder
2. The knife carrier and the knife
3. Screws and device
4. Flywheel
5. Base

Core of Microtome / Maintenance of Microtome:

1. After cutting sections on the microtome , all accumulated paraffin and tissue should be brushed away with a soft brush.
2. All metal parts may be wiped clean with xylence.
3. Avoid continuous supplication of xylence to the rest of the machine.
4. The machine must be dried carefully.
5. The microtome should be covered when not in use