Ejection: pulas and Shock
Each piptte is fitted with a tip ejector system to
eliminate the risk of contamination the tip ejector system is consist of-
·Ejector plunger
·Spring
·Spring support
·Tip ejector bushing.
The
plunger is pressed by them to eject the tip.
Techniques:
1.A clean tip should
firmly pushed on to the tip cone of the pipette.
2.Wet the newly attached
tip with the solution being pipette before any actual pupating takes place.
This is done by filling and emptying the tip 2-3 times.
3.Hold the pipette
verticattly during the liquid in take with the grippe resting on index finger.
4.The temperature of the
tip and pipette should be equalized to the solution.
Forward technique:
1.Depress the operation
button to the first stop.
2.Dip the tip under the
surface of the liquid in the reservoir about I can deep and slowly release the
button withdraw the tip from the liquid touching against the edge of the
reservoir to remove excess liquid.
3.Deliver the liquid
gently depressing the operating button to the first stop. After a delay of one
second continue depressing the button down to the second sop.
4.Release the button.
Reverse technique:
Reverse
technique is used for high discus fluid with tendency to foam easily or for
small volume.
1.Depress the button up
to second stop .
2.Dip the tip under the
surface of the liquid in the reservoir about I cm deep and slowly release the
button withdraw the tip from the liquid by touching against the edge of the
reservoir to remove excess liquid.
3.Deliver the present
volume by depressing up to first stop and hold it. the remaining should not be
included
Repetitive technique:
For rapid and simple repeated delivery
1.Depress the button up
to second stop.
2.Dip the tip under the
surface of the liquid in the reservoir about cm deep and slowly release the
button withdraw the tip tromp the liquid by touching against the edge of the
reservoir to remove excess liquid.
3.Deliver the liquid
gently depressing the operating button to the first stop .After a delay of 1
second continue depressing the button down to the second stop.
4.Continue second and
third step.
Pulse:
Pulse is a rhythmic dilatation and elongation of arterial wall as a result of
pressure changes created by the intermittent ejection of blood from heart to
the already full aorta feeding the arterial system, transmit as a wave to the
periphery.
Range :
60-90%/min
Average:72/min.
Shock:
It is
an abnormal physiological condition resulting from inadequate population of
blood to the aorta thus causes inadequate blood flow perusing the capillaries
of tissues and organs.
Type:
1.Hypovolemic shock.
a.Hemorrhagic shock
b.Traumatic shock
c.Burn shock.
2.Carcinogenic shock.
3.Low resistance shock.
4.Anaphylactic shock
Glassware
refers to a variety of equipment traditionally use for scientific experiments
taste procedure and other work in the laboratory,
Staining: Staining is an auxiliary technique used in microscopy to
enhance contrast in the microscopic image ,it is also a procedure colorization
of cells and other.